Post by LymeEnigma on Jun 3, 2008 8:57:55 GMT -8
Beta-amyloid deposition and Alzheimer's type changes induced by Borrelia spirochetes
Judith Miklossya, b, Corresponding Author Contact Information, E-mail The Corresponding Author, Andras Kisc, Alexandra Radenovicc, Lisa Millerd, Laszlo Forroc, Ralph Martinse, Krzysztof Reissf, Nune Darbinianf, Pushpa Darekarb, Laszlo Mihalyg and Kamel Khalili
Abstract:
The pathological hallmarks of Alzheimer's disease (AD) consist of β-amyloid plaques and neurofibrillary tangles in affected brain areas. The processes, which drive this host reaction are unknown. To determine whether an analogous host reaction to that occurring in AD could be induced by infectious agents, we exposed mammalian glial and neuronal cells in vitro to Borrelia burgdorferi spirochetes and to the inflammatory bacterial lipopolysaccharide (LPS). Morphological changes analogous to the amyloid deposits of AD brain were observed following 2–8 weeks of exposure to the spirochetes. Increased levels of β-amyloid presursor protein (AβPP) and hyperphosphorylated tau were also detected by Western blots of extracts of cultured cells that had been treated with spirochetes or LPS. These observations indicate that, by exposure to bacteria or to their toxic products, host responses similar in nature to those observed in AD may be induced.
www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T09-4G5BJ7S-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=47b89ea7bb5d63df414830564e0d79de
Judith Miklossya, b, Corresponding Author Contact Information, E-mail The Corresponding Author, Andras Kisc, Alexandra Radenovicc, Lisa Millerd, Laszlo Forroc, Ralph Martinse, Krzysztof Reissf, Nune Darbinianf, Pushpa Darekarb, Laszlo Mihalyg and Kamel Khalili
Abstract:
The pathological hallmarks of Alzheimer's disease (AD) consist of β-amyloid plaques and neurofibrillary tangles in affected brain areas. The processes, which drive this host reaction are unknown. To determine whether an analogous host reaction to that occurring in AD could be induced by infectious agents, we exposed mammalian glial and neuronal cells in vitro to Borrelia burgdorferi spirochetes and to the inflammatory bacterial lipopolysaccharide (LPS). Morphological changes analogous to the amyloid deposits of AD brain were observed following 2–8 weeks of exposure to the spirochetes. Increased levels of β-amyloid presursor protein (AβPP) and hyperphosphorylated tau were also detected by Western blots of extracts of cultured cells that had been treated with spirochetes or LPS. These observations indicate that, by exposure to bacteria or to their toxic products, host responses similar in nature to those observed in AD may be induced.
www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T09-4G5BJ7S-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=47b89ea7bb5d63df414830564e0d79de