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Post by enochroot on Jul 28, 2008 11:03:28 GMT -8
He only ran the IGG, as I have been at this a year.
Will let you know about the other tests later!
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Post by LymeEnigma on Jul 28, 2008 21:12:14 GMT -8
Modern medicine at its best....  |
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Post by LymeEnigma on Jul 28, 2008 21:14:05 GMT -8
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hannahd
Searching for Answers
Posts: 12
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Post by hannahd on Aug 1, 2008 19:31:40 GMT -8
My western blot was positive but I don't know what bands showed.
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Post by enochroot on Aug 7, 2008 11:53:33 GMT -8
checked again - they did run IGM on the "Lyme bands" 23 41 and another -
all negative.
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Post by LymeEnigma on Aug 7, 2008 12:02:16 GMT -8
Well, while false negatives are always possible, it is also possible that you are now dealing with something other than Lyme.... I'm currently at a loss, regarding my symptoms; suddenly I feel great, physically ... but I now can't sleep and my anxiety is through the roof.
This puzzle is just too big, I fear....
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Post by enochroot on Aug 12, 2008 15:31:51 GMT -8
Interesting UK article  ============================================== Potentially misleading Western blot results in Lyme disease diagnosis Mavin, S The laboratory diagnosis of Lyme disease is complex1 and serology remains the technique of choice. Recommended practice is a two-step process involving a sensitive screening enzyme immunoassay (EIA) followed by a more specific confirmatory Western blot for all EIA-positive and equivocal samples and for EIA-negative samples with a high clinical suspicion of Lyme disease (e.g., tick bite and erythema migrans).1,2 However, Western blot results require careful interpretation. The National Lyme Disease Testing Service Laboratory, Raigmore Hospital, Inverness, tests over 3000 samples annually from across Scotland, many of which are from complex clinical cases. It was noted recently that serum from a patient with confirmed parvovirus B19 infection crossreacted with the in-house Borrelia burgdorferi IgG Western blot. This could lead to the wrong interpretation of Western blot results. This study aims to discover if other viral infections produce similar results with the B. burgdorferi IgG Western blot. The study group consisted of six patients found to contain IgM antibodies to parvovirus B19 infection (parvovirus B19 IgM EIA, Biotrin International), six with cytomegalovirus (CMV) IgM (CMV IgM EIA, Microgen Bioproducts) and six with Epstein-Barr virus (EBV) IgM (EBV VCA IgM EIA, Diasorin). All patients had good clinical evidence of viral infection (Table 1). The 18 serum samples were analysed by commercial B. burgdorferi IgG/IgM EIA (Zeus Scientific, New Jersey, USA) and an in-house B. burgdorferi IgG Western blot. The EIA was performed according to the manufacturer's instructions and the Western blot was performed as described previously.2 The blots used contained sodium dodecyl sulphate-extracted B. burgdorferi (i.e., antigen). A commercial positive control (Zeus Scientific) and in-house positive control, with defined band patterns, were included in each blot run. The number, intensity and molecular weight of bands observed with each sample were recorded, and a negative, equivocal, weak positive or positive result assigned according to predetermined criteria.3 The Western blot was repeated for all samples. Previous or subsequent serum samples from five patients (serum samples 5,8. 9,11 and 12 |
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Post by LymeEnigma on Aug 13, 2008 11:25:39 GMT -8
Great find, Enoch!!!
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Post by enochroot on Aug 13, 2008 15:07:15 GMT -8
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Post by enochroot on Aug 14, 2008 13:14:46 GMT -8
Put a post on Healingwell about getting six western blots over
a year all negative, just to see what people had to say...
Got one reply saying "Quest western blots don't count"
If I can scrape up the money for Igenex, I am not sure my doc wil
go for it as in writing me a script to draw blood for the kit.
He was skeptical back when I did the Central Florida Research test.
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Post by LymeEnigma on Aug 15, 2008 9:55:18 GMT -8
I really don't know what I think about Quest and Igenex; I'm not really sure I trust either.
I had one vial of blood sent to Quest, and that one sample produced a positive ELISA and a very negative Western blot (how does blood with the antibodies to produce a positive ELISA produce a Western blot with zero bands, unless someone made a gross mistake on one of them?!); a repeat Quest Western blot lit up for band 41kDa only. About a year later I sent for an Igenex test kit and convinced my doctor to comply. The blood sent out for that produced a negative ELISA and a positive Western blot. Once again, how does one vial of blood, used at the same lab, produce one positive and one negative, unless 1) the tests are unreliable, or 2) the person/people performing the tests are inept?
Something just isn't right with that; I wish I knew which were the false readings, but that's just another guessing game in itself ... unfortunately, instead of getting any real answers with the further blood tests, I only have more questions.
If you have the money to do the tests, I'd say go ahead ... but if the tests will burden you financially, I'd say pass.
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Post by LymeEnigma on Aug 24, 2008 10:00:34 GMT -8
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